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2021
Anbumani, Silambarasan; Silva, Aldeliane M.; Carvalho, Isis G. B.; Fischer, Eduarda Regina; Silva, Mariana; Zuben, Antonio Augusto G.; Carvalho, Hernandes F.; Souza, Alessandra A.; Janissen, Richard; Cotta, Monica A.
Controlled spatial organization of bacterial growth reveals key role of cell filamentation preceding Xylella fastidiosa biofilm formation Journal Article
In: npj Biofilms and Microbiomes 2021 7:1, vol. 7, iss. 1, pp. 1-12, 2021, ISSN: 2055-5008.
Abstract | Links | BibTeX | Tags: Biofilms, Cellular microbiology, Pathogens
@article{Anbumani2021,
title = {Controlled spatial organization of bacterial growth reveals key role of cell filamentation preceding Xylella fastidiosa biofilm formation},
author = {Silambarasan Anbumani and Aldeliane M. Silva and Isis G. B. Carvalho and Eduarda Regina Fischer and Mariana Silva and Antonio Augusto G. Zuben and Hernandes F. Carvalho and Alessandra A. Souza and Richard Janissen and Monica A. Cotta},
url = {https://www.nature.com/articles/s41522-021-00258-9},
doi = {10.1038/s41522-021-00258-9},
issn = {2055-5008},
year = {2021},
date = {2021-01-01},
journal = {npj Biofilms and Microbiomes 2021 7:1},
volume = {7},
issue = {1},
pages = {1-12},
publisher = {Nature Publishing Group},
abstract = {The morphological plasticity of bacteria to form filamentous cells commonly represents an adaptive strategy induced by stresses. In contrast, for diverse human and plant pathogens, filamentous cells have been recently observed during biofilm formation, but their functions and triggering mechanisms remain unclear. To experimentally identify the underlying function and hypothesized cell communication triggers of such cell morphogenesis, spatially controlled cell patterning is pivotal. Here, we demonstrate highly selective cell adhesion of the biofilm-forming phytopathogen Xylella fastidiosa to gold-patterned SiO2 substrates with well-defined geometries and dimensions. The consequent control of both cell density and distances between cell clusters demonstrated that filamentous cell formation depends on cell cluster density, and their ability to interconnect neighboring cell clusters is distance-dependent. This process allows the creation of large interconnected cell clusters that form the structural framework for macroscale biofilms. The addition of diffusible signaling molecules from supernatant extracts provides evidence that cell filamentation is induced by quorum sensing. These findings and our innovative platform could facilitate therapeutic developments targeting biofilm formation mechanisms of X. fastidiosa and other pathogens.},
keywords = {Biofilms, Cellular microbiology, Pathogens},
pubstate = {published},
tppubtype = {article}
}
The morphological plasticity of bacteria to form filamentous cells commonly represents an adaptive strategy induced by stresses. In contrast, for diverse human and plant pathogens, filamentous cells have been recently observed during biofilm formation, but their functions and triggering mechanisms remain unclear. To experimentally identify the underlying function and hypothesized cell communication triggers of such cell morphogenesis, spatially controlled cell patterning is pivotal. Here, we demonstrate highly selective cell adhesion of the biofilm-forming phytopathogen Xylella fastidiosa to gold-patterned SiO2 substrates with well-defined geometries and dimensions. The consequent control of both cell density and distances between cell clusters demonstrated that filamentous cell formation depends on cell cluster density, and their ability to interconnect neighboring cell clusters is distance-dependent. This process allows the creation of large interconnected cell clusters that form the structural framework for macroscale biofilms. The addition of diffusible signaling molecules from supernatant extracts provides evidence that cell filamentation is induced by quorum sensing. These findings and our innovative platform could facilitate therapeutic developments targeting biofilm formation mechanisms of X. fastidiosa and other pathogens.
2018
Silva, Aldeliane M.; Sahoo, Prasana K.; Cavalli, Alessandro; Souza, Alessandra A.; Bakkers, Erik P. A. M.; Cesar, Carlos L.; Janissen, Richard; Cotta, Monica A.
In: Small Methods, vol. 2, iss. 7, pp. 1700411, 2018, ISSN: 2366-9608.
Abstract | Links | BibTeX | Tags: Biofilms, cell adhesion forces, confocal microscopy, nanowires, Xylella fastidiosa
@article{nokey,
title = {Nanowire Arrays as Force Sensors with Super-Resolved Localization Position Detection: Application to Optical Measurement of Bacterial Adhesion Forces},
author = {Aldeliane M. Silva and Prasana K. Sahoo and Alessandro Cavalli and Alessandra A. Souza and Erik P. A. M. Bakkers and Carlos L. Cesar and Richard Janissen and Monica A. Cotta},
url = {https://onlinelibrary.wiley.com/doi/full/10.1002/smtd.201700411 https://onlinelibrary.wiley.com/doi/abs/10.1002/smtd.201700411 https://onlinelibrary.wiley.com/doi/10.1002/smtd.201700411},
doi = {10.1002/SMTD.201700411},
issn = {2366-9608},
year = {2018},
date = {2018-01-01},
journal = {Small Methods},
volume = {2},
issue = {7},
pages = {1700411},
publisher = {John Wiley & Sons, Ltd},
abstract = {The design and application of indium phosphide (InP) nanowire arrays to acquire Xylella fastidiosa bacterial cell vector force maps are discussed. The nanowire deflections are measured with subdiffraction localization confocal laser scanning microscopy (CLSM). The nanowire mechanical stability in air and liquid media as well as methods to average out thermally induced oscillations are investigated. The accuracy of center determination of the CLSM reflected laser intensity profile at nanowire apex is studied using Gaussian fitting and localization microscopy techniques. These results show that the method is reliable for measuring nanowire displacements above ≈25 nm. Corresponding force ranges probed by this method can be customized depending on nanowire geometry and array configuration. The method is applied to explore X. fastidiosa cell adhesion forces on the InP nanowire surface, and in situ probes the effect of N-acetylcysteine on adhered cells. Future perspectives for application of this method in microbiology studies are also outlined.},
keywords = {Biofilms, cell adhesion forces, confocal microscopy, nanowires, Xylella fastidiosa},
pubstate = {published},
tppubtype = {article}
}
The design and application of indium phosphide (InP) nanowire arrays to acquire Xylella fastidiosa bacterial cell vector force maps are discussed. The nanowire deflections are measured with subdiffraction localization confocal laser scanning microscopy (CLSM). The nanowire mechanical stability in air and liquid media as well as methods to average out thermally induced oscillations are investigated. The accuracy of center determination of the CLSM reflected laser intensity profile at nanowire apex is studied using Gaussian fitting and localization microscopy techniques. These results show that the method is reliable for measuring nanowire displacements above ≈25 nm. Corresponding force ranges probed by this method can be customized depending on nanowire geometry and array configuration. The method is applied to explore X. fastidiosa cell adhesion forces on the InP nanowire surface, and in situ probes the effect of N-acetylcysteine on adhered cells. Future perspectives for application of this method in microbiology studies are also outlined.
Silva, Aldeliane M.; Sahoo, Prasana K.; Cavalli, Alessandro; Souza, Alessandra A.; Bakkers, Erik P. A. M.; Cesar, Carlos L.; Janissen, Richard; Cotta, Monica A.
In: Small Methods, vol. 2, iss. 7, pp. 1700411, 2018, ISSN: 2366-9608.
Abstract | Links | BibTeX | Tags: Biofilms, cell adhesion forces, confocal microscopy, nanowires, Xylella fastidiosa
@article{nokey,
title = {Nanowire Arrays as Force Sensors with Super-Resolved Localization Position Detection: Application to Optical Measurement of Bacterial Adhesion Forces},
author = {Aldeliane M. Silva and Prasana K. Sahoo and Alessandro Cavalli and Alessandra A. Souza and Erik P. A. M. Bakkers and Carlos L. Cesar and Richard Janissen and Monica A. Cotta},
url = {https://onlinelibrary.wiley.com/doi/full/10.1002/smtd.201700411 https://onlinelibrary.wiley.com/doi/abs/10.1002/smtd.201700411 https://onlinelibrary.wiley.com/doi/10.1002/smtd.201700411},
doi = {10.1002/SMTD.201700411},
issn = {2366-9608},
year = {2018},
date = {2018-01-01},
journal = {Small Methods},
volume = {2},
issue = {7},
pages = {1700411},
publisher = {John Wiley & Sons, Ltd},
abstract = {The design and application of indium phosphide (InP) nanowire arrays to acquire Xylella fastidiosa bacterial cell vector force maps are discussed. The nanowire deflections are measured with subdiffraction localization confocal laser scanning microscopy (CLSM). The nanowire mechanical stability in air and liquid media as well as methods to average out thermally induced oscillations are investigated. The accuracy of center determination of the CLSM reflected laser intensity profile at nanowire apex is studied using Gaussian fitting and localization microscopy techniques. These results show that the method is reliable for measuring nanowire displacements above ≈25 nm. Corresponding force ranges probed by this method can be customized depending on nanowire geometry and array configuration. The method is applied to explore X. fastidiosa cell adhesion forces on the InP nanowire surface, and in situ probes the effect of N-acetylcysteine on adhered cells. Future perspectives for application of this method in microbiology studies are also outlined.},
keywords = {Biofilms, cell adhesion forces, confocal microscopy, nanowires, Xylella fastidiosa},
pubstate = {published},
tppubtype = {article}
}
The design and application of indium phosphide (InP) nanowire arrays to acquire Xylella fastidiosa bacterial cell vector force maps are discussed. The nanowire deflections are measured with subdiffraction localization confocal laser scanning microscopy (CLSM). The nanowire mechanical stability in air and liquid media as well as methods to average out thermally induced oscillations are investigated. The accuracy of center determination of the CLSM reflected laser intensity profile at nanowire apex is studied using Gaussian fitting and localization microscopy techniques. These results show that the method is reliable for measuring nanowire displacements above ≈25 nm. Corresponding force ranges probed by this method can be customized depending on nanowire geometry and array configuration. The method is applied to explore X. fastidiosa cell adhesion forces on the InP nanowire surface, and in situ probes the effect of N-acetylcysteine on adhered cells. Future perspectives for application of this method in microbiology studies are also outlined.