Publications

@article{Mauricio2019,

title = {Analysis of defense-related gene expression in citrus hybrids infected by xylella fastidiosa},

author = {F. N. Mauricio and T. A. T. Soratto and J. A. Diogo and R. L. Boscariol-Camargo and A. A. De Souza and H. D. Coletta-Filho and J. A. A. Silva and A. H. Medeiros and M. A. Machado and M. Cristofani-Yaly},

url = {https://apsjournals.apsnet.org/doi/10.1094/PHYTO-09-18-0366-FI},

doi = {10.1094/PHYTO-09-18-0366-FI/ASSET/IMAGES/LARGE/PHYTO-09-18-0366-FI_F3.JPEG},

issn = {0031949X},

year  = {2019},

date = {2019-01-01},

journal = {Phytopathology},

volume = {109},

issue = {2},

pages = {301-306},

publisher = {American Phytopathological Society},

abstract = {Resistance to Xylella fastidiosa was evaluated in 264 hybrids of crosses between Murcott tangor (Citrus reticulata × Citrus sinensis) and Pera sweet orange (C. sinensis) under field conditions. Uninfected hybrids were grafted with buds collected from Pera sweet orange plants infected with X. fastidiosa, forming a plant with two scions (i.e., hybrid branches and Pera sweet orange branches). From these plants, we chose 10 genotypes with three biological replicates. We evaluated gene expression, bacterial multiplication, and citrus variegated chlorosis (CVC) symptom development in both scions. X. fastidiosa was not detected in most hybrid scions and none showed disease symptoms. In contrast, all Pera sweet orange scions were infected with X. fastidiosa and expressed symptoms of CVC. We quantified the expression of 12 defense-related genes by qPCR comparing resistant to susceptible scions. We suggest that some of these genes are involved in resistance of the hybrids to X. fastidiosa, since their expression was significantly higher in the resistant hybrid scions than in tolerant hybrids and scions originated from CVC symptomatic Pera sweet orange buds. However, we note that these data should be interpreted carefully, as the plant genotypes tested are related but necessarily distinct (hybrids of C. reticulata and C. sinensis, in relation to a C. sinensis control). A principal component analysis revealed a relationship between the expression of these genes and hybrid scions, and between scions that originated from infected buds and the presence of the bacteria and plant symptoms. Multiyear field trials are necessary to develop plant resistance to X. fastidiosa. While the experimental design used here had limitations, it allowed us to identify a set of genes potentially involved in Citrus sp. resistance to this pathogen. Future work on the role of these genes in plant defenses to X. fastidiosa infection is necessary to confirm their importance in the displayed resistance phenotype.},

keywords = {and eighth authors: Universidade Federal de São Carlos, and seventh author: Pólo Regional Alta Mogiana, and tenth authors: Centro de Citricultura Sylvio Moreira/IAC, Araras, Brazil, Brazil 13490-970, Campus Araras, Colina, Cordeirópolis, CP 35, CP04, fifth, First, fourth, Km 174, ninth, Rodovia Anhanguera, São Paulo, second, sixth, SP, third},

pubstate = {published},

tppubtype = {article}

}

@article{nokey,

title = {Citrus leprosis virus N: A new dichorhavirus causing Citrus leprosis disease},

author = {Pedro Luis Ramos-González and Camila Chabi-Jesus and Orlene Guerra-Peraza and Aline Daniele Tassi and Elliot Watanabe Kitajima and Ricardo Harakava and Renato Barbosa Salaroli and Juliana Freitas-Astúa},

url = {https://apsjournals.apsnet.org/doi/10.1094/PHYTO-02-17-0042-R},

doi = {10.1094/PHYTO-02-17-0042-R/ASSET/IMAGES/LARGE/PHYTO-02-17-0042-R_F8.JPEG},

issn = {0031949X},

year  = {2017},

date = {2017-01-01},

journal = {Phytopathology},

volume = {107},

issue = {8},

pages = {963-976},

publisher = {American Phytopathological Society},

abstract = {Citrus leprosis (CL) is a viral disease endemic to theWestern Hemisphere that produces local necrotic and chlorotic lesions on leaves, branches, and fruit and causes serious yield reduction in citrus orchards. Samples of sweet orange (Citrus × sinensis) trees showing CL symptoms were collected during a survey in noncommercial citrus areas in the southeast region of Brazil in 2013 to 2016. Transmission electron microscopy analyses of foliar lesions confirmed the presence of rod-like viral particles commonly associated with CL in the nucleus and cytoplasm of infected cells. However, every attempt to identify these particles by reverse-transcription polymerase chain reaction tests failed, even though all described primers for the detection of known CL-causing cileviruses and dichorhaviruses were used. Next-generation sequencing of total RNA extracts from three symptomatic samples revealed the genome of distinct, although highly related (>92% nucleotide sequence identity), viruses whose genetic organization is similar to that of dichorhaviruses. The genome sequence of these viruses showed <62% nucleotide sequence identity with those of orchid fleck virus and coffee ringspot virus. Globally, the deduced amino acid sequences of the open reading frames they encode share 32.7 to 63.8% identity with the proteins of the dichorhavirids. Mites collected from both the naturally infected citrus trees and those used for the transmission of one of the characterized isolates to Arabidopsis plants were anatomically recognized as Brevipalpus phoenicis sensu stricto. Molecular and biological features indicate that the identified viruses belong to a new species of CL-associated dichorhavirus, which we propose to call Citrus leprosis N dichorhavirus. Our results, while emphasizing the increasing diversity of viruses causing CL disease, lead to a reevaluation of the nomenclature of those viruses assigned to the genus Dichorhavirus. In this regard, a comprehensive discussion is presented.},

keywords = {and eighth authors: Lab Bioquímica Fitopatológica, and seventh authors:, Brazil; second, fifth, First, fourth, Instituto Biológico, São Paulo 04014-002, second, sixth, third},

pubstate = {published},

tppubtype = {article}

}

@article{nokey,

title = {Citrus leprosis virus N: A new dichorhavirus causing Citrus leprosis disease},

author = {Pedro Luis Ramos-González and Camila Chabi-Jesus and Orlene Guerra-Peraza and Aline Daniele Tassi and Elliot Watanabe Kitajima and Ricardo Harakava and Renato Barbosa Salaroli and Juliana Freitas-Astúa},

url = {https://apsjournals.apsnet.org/doi/10.1094/PHYTO-02-17-0042-R},

doi = {10.1094/PHYTO-02-17-0042-R/ASSET/IMAGES/LARGE/PHYTO-02-17-0042-R_F8.JPEG},

issn = {0031949X},

year  = {2017},

date = {2017-01-01},

journal = {Phytopathology},

volume = {107},

issue = {8},

pages = {963-976},

publisher = {American Phytopathological Society},

abstract = {Citrus leprosis (CL) is a viral disease endemic to theWestern Hemisphere that produces local necrotic and chlorotic lesions on leaves, branches, and fruit and causes serious yield reduction in citrus orchards. Samples of sweet orange (Citrus × sinensis) trees showing CL symptoms were collected during a survey in noncommercial citrus areas in the southeast region of Brazil in 2013 to 2016. Transmission electron microscopy analyses of foliar lesions confirmed the presence of rod-like viral particles commonly associated with CL in the nucleus and cytoplasm of infected cells. However, every attempt to identify these particles by reverse-transcription polymerase chain reaction tests failed, even though all described primers for the detection of known CL-causing cileviruses and dichorhaviruses were used. Next-generation sequencing of total RNA extracts from three symptomatic samples revealed the genome of distinct, although highly related (>92% nucleotide sequence identity), viruses whose genetic organization is similar to that of dichorhaviruses. The genome sequence of these viruses showed <62% nucleotide sequence identity with those of orchid fleck virus and coffee ringspot virus. Globally, the deduced amino acid sequences of the open reading frames they encode share 32.7 to 63.8% identity with the proteins of the dichorhavirids. Mites collected from both the naturally infected citrus trees and those used for the transmission of one of the characterized isolates to Arabidopsis plants were anatomically recognized as Brevipalpus phoenicis sensu stricto. Molecular and biological features indicate that the identified viruses belong to a new species of CL-associated dichorhavirus, which we propose to call Citrus leprosis N dichorhavirus. Our results, while emphasizing the increasing diversity of viruses causing CL disease, lead to a reevaluation of the nomenclature of those viruses assigned to the genus Dichorhavirus. In this regard, a comprehensive discussion is presented.},

keywords = {and eighth authors: Lab Bioquímica Fitopatológica, and seventh authors:, Brazil; second, fifth, First, fourth, Instituto Biológico, São Paulo 04014-002, second, sixth, third},

pubstate = {published},

tppubtype = {article}

}

@article{nokey,

title = {Citrus leprosis virus N: A new dichorhavirus causing Citrus leprosis disease},

author = {Pedro Luis Ramos-González and Camila Chabi-Jesus and Orlene Guerra-Peraza and Aline Daniele Tassi and Elliot Watanabe Kitajima and Ricardo Harakava and Renato Barbosa Salaroli and Juliana Freitas-Astúa},

url = {https://apsjournals.apsnet.org/doi/10.1094/PHYTO-02-17-0042-R},

doi = {10.1094/PHYTO-02-17-0042-R/ASSET/IMAGES/LARGE/PHYTO-02-17-0042-R_F8.JPEG},

issn = {0031949X},

year  = {2017},

date = {2017-01-01},

journal = {Phytopathology},

volume = {107},

issue = {8},

pages = {963-976},

publisher = {American Phytopathological Society},

abstract = {Citrus leprosis (CL) is a viral disease endemic to theWestern Hemisphere that produces local necrotic and chlorotic lesions on leaves, branches, and fruit and causes serious yield reduction in citrus orchards. Samples of sweet orange (Citrus × sinensis) trees showing CL symptoms were collected during a survey in noncommercial citrus areas in the southeast region of Brazil in 2013 to 2016. Transmission electron microscopy analyses of foliar lesions confirmed the presence of rod-like viral particles commonly associated with CL in the nucleus and cytoplasm of infected cells. However, every attempt to identify these particles by reverse-transcription polymerase chain reaction tests failed, even though all described primers for the detection of known CL-causing cileviruses and dichorhaviruses were used. Next-generation sequencing of total RNA extracts from three symptomatic samples revealed the genome of distinct, although highly related (>92% nucleotide sequence identity), viruses whose genetic organization is similar to that of dichorhaviruses. The genome sequence of these viruses showed <62% nucleotide sequence identity with those of orchid fleck virus and coffee ringspot virus. Globally, the deduced amino acid sequences of the open reading frames they encode share 32.7 to 63.8% identity with the proteins of the dichorhavirids. Mites collected from both the naturally infected citrus trees and those used for the transmission of one of the characterized isolates to Arabidopsis plants were anatomically recognized as Brevipalpus phoenicis sensu stricto. Molecular and biological features indicate that the identified viruses belong to a new species of CL-associated dichorhavirus, which we propose to call Citrus leprosis N dichorhavirus. Our results, while emphasizing the increasing diversity of viruses causing CL disease, lead to a reevaluation of the nomenclature of those viruses assigned to the genus Dichorhavirus. In this regard, a comprehensive discussion is presented.},

keywords = {and eighth authors: Lab Bioquímica Fitopatológica, and seventh authors:, Brazil; second, fifth, First, fourth, Instituto Biológico, São Paulo 04014-002, second, sixth, third},

pubstate = {published},

tppubtype = {article}

}