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2017
Arena, Gabriella Dias; Ramos-González, Pedro Luis; Nunes, Maria Andréia; Jesus, Camila Chabi; Calegario, Renata Faier; Kitajima, Elliot Watanabe; Novelli, Valdenice Moreira; Freitas-Astúa, Juliana
Arabidopsis thaliana as a model host for Brevipalpus mite-transmitted viruses Journal Article
In: Scientia Agricola, vol. 74, iss. 1, pp. 85-89, 2017, ISSN: 1678-992X.
Abstract | Links | BibTeX | Tags: Cilevirus, Clerodendrum chlorotic spot virus, Coffee ringspot virus, Dichorhavirus, Solanum violaefolium ringspot virus
@article{Arena2017,
title = {\textit{Arabidopsis thaliana} as a model host for \textit{Brevipalpus} mite-transmitted viruses},
author = {Gabriella Dias Arena and Pedro Luis Ramos-González and Maria Andréia Nunes and Camila Chabi Jesus and Renata Faier Calegario and Elliot Watanabe Kitajima and Valdenice Moreira Novelli and Juliana Freitas-Astúa},
url = {http://www.scielo.br/j/sa/a/mLKYNzqSqGsJZwmP5XM5LbH/?lang=en},
doi = {10.1590/1678-992X-2015-0380},
issn = {1678-992X},
year = {2017},
date = {2017-01-01},
journal = {Scientia Agricola},
volume = {74},
issue = {1},
pages = {85-89},
publisher = {Escola Superior de Agricultura "Luiz de Queiroz"},
abstract = {ABSTRACT: Brevipalpus-transmitted viruses (BTV) are a taxonomically diverse group of plant viruses which severely affect a number of major crops. Members of the group can be sub-classified into cytoplasmic (BTV-C) or nuclear type (BTV-N) according to the accumulation sites of virions in the infected plant cells. Both types of BTV produce only local infections near the point of inoculation by viruliferous mites. Features of BTV-plant interactions such as the failure of systemic spread in their natural hosts are poorly understood. In this study we evaluated Arabidopsis thaliana, a model plant commonly used for the study of plant-virus interactions, as an alternative host for BTV. Infection of Arabidopsis with the BTV-N Coffee ringspot virus and Clerodendrum chlorotic spot virus, and the BTV-C Solanum violaefolium ringspot virus, were mediated by viruliferous Brevipalpus mites collected in the wild. Upon infestation, local lesions appeared in 7 to 10 days on leaves of, at least, 80 % of the assayed plants. Presence of viral particles and characteristic cytopathic effects were detected by transmission electron microscopy (TEM) and the viral identities confirmed by specific reverse-transcriptase polymerase chain reaction (RT-PCR) and further amplicon sequencing. The high infection rate and reproducibility of symptoms of the three different viruses assayed validate A. thaliana as a feasible alternative experimental host for BTV.},
keywords = {Cilevirus, Clerodendrum chlorotic spot virus, Coffee ringspot virus, Dichorhavirus, Solanum violaefolium ringspot virus},
pubstate = {published},
tppubtype = {article}
}
ABSTRACT: Brevipalpus-transmitted viruses (BTV) are a taxonomically diverse group of plant viruses which severely affect a number of major crops. Members of the group can be sub-classified into cytoplasmic (BTV-C) or nuclear type (BTV-N) according to the accumulation sites of virions in the infected plant cells. Both types of BTV produce only local infections near the point of inoculation by viruliferous mites. Features of BTV-plant interactions such as the failure of systemic spread in their natural hosts are poorly understood. In this study we evaluated Arabidopsis thaliana, a model plant commonly used for the study of plant-virus interactions, as an alternative host for BTV. Infection of Arabidopsis with the BTV-N Coffee ringspot virus and Clerodendrum chlorotic spot virus, and the BTV-C Solanum violaefolium ringspot virus, were mediated by viruliferous Brevipalpus mites collected in the wild. Upon infestation, local lesions appeared in 7 to 10 days on leaves of, at least, 80 % of the assayed plants. Presence of viral particles and characteristic cytopathic effects were detected by transmission electron microscopy (TEM) and the viral identities confirmed by specific reverse-transcriptase polymerase chain reaction (RT-PCR) and further amplicon sequencing. The high infection rate and reproducibility of symptoms of the three different viruses assayed validate A. thaliana as a feasible alternative experimental host for BTV.