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Check the list of papers from NScTI Citrus
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2017
Galdeano, Diogo Manzano; Breton, Michèle Claire; Lopes, João Roberto Spotti; Falk, Bryce W.; Machado, Marcos Antonio
Oral delivery of double-stranded RNAs induces mortality in nymphs and adults of the Asian citrus psyllid, Diaphorina citri Journal Article
In: PLOS ONE, vol. 12, iss. 3, pp. e0171847, 2017, ISSN: 1932-6203.
Abstract | Links | BibTeX | Tags: Apoptosis, Chitin, Death rates, Diet, Insects, Leaves, Nymphs, RNA interference
@article{Galdeano2017,
title = {Oral delivery of double-stranded RNAs induces mortality in nymphs and adults of the Asian citrus psyllid, Diaphorina citri},
author = {Diogo Manzano Galdeano and Michèle Claire Breton and João Roberto Spotti Lopes and Bryce W. Falk and Marcos Antonio Machado},
url = {https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0171847},
doi = {10.1371/JOURNAL.PONE.0171847},
issn = {1932-6203},
year = {2017},
date = {2017-01-01},
journal = {PLOS ONE},
volume = {12},
issue = {3},
pages = {e0171847},
publisher = {Public Library of Science},
abstract = {The Asian citrus psyllid (ACP), Diaphorina citri Kuwayama, is one of the most important citrus pests. ACP is the vector of the phloem-limited bacteria Candidatus Liberibacter americanus and Candidatus Liberibacter asiaticus, the causal agents of the devastating citrus disease huanglongbing (HLB). The management of HLB is based on the use of healthy young plants, eradication of infected plants and chemical control of the vector. RNA interference (RNAi) has proven to be a promising tool to control pests and explore gene functions. Recently, studies have reported that target mRNA knockdown in many insects can be induced through feeding with double-stranded RNA (dsRNA). In the current study, we targeted the cathepsin D, chitin synthase and inhibitor of apoptosis genes of adult and nymph ACP by feeding artificial diets mixed with dsRNAs and Murraya paniculata leaves placed in dsRNAs solutions, respectively. Adult ACP mortality was positively correlated with the amount of dsRNA used. Both nymphs and adult ACP fed dsRNAs exhibited significantly increased mortality over time compared with that of the controls. Moreover, qRT-PCR analysis confirmed the dsRNA-mediated RNAi effects on target mRNAs. These results showed that RNAi can be a powerful tool for gene function studies in ACP and perhaps for HLB control.},
keywords = {Apoptosis, Chitin, Death rates, Diet, Insects, Leaves, Nymphs, RNA interference},
pubstate = {published},
tppubtype = {article}
}
The Asian citrus psyllid (ACP), Diaphorina citri Kuwayama, is one of the most important citrus pests. ACP is the vector of the phloem-limited bacteria Candidatus Liberibacter americanus and Candidatus Liberibacter asiaticus, the causal agents of the devastating citrus disease huanglongbing (HLB). The management of HLB is based on the use of healthy young plants, eradication of infected plants and chemical control of the vector. RNA interference (RNAi) has proven to be a promising tool to control pests and explore gene functions. Recently, studies have reported that target mRNA knockdown in many insects can be induced through feeding with double-stranded RNA (dsRNA). In the current study, we targeted the cathepsin D, chitin synthase and inhibitor of apoptosis genes of adult and nymph ACP by feeding artificial diets mixed with dsRNAs and Murraya paniculata leaves placed in dsRNAs solutions, respectively. Adult ACP mortality was positively correlated with the amount of dsRNA used. Both nymphs and adult ACP fed dsRNAs exhibited significantly increased mortality over time compared with that of the controls. Moreover, qRT-PCR analysis confirmed the dsRNA-mediated RNAi effects on target mRNAs. These results showed that RNAi can be a powerful tool for gene function studies in ACP and perhaps for HLB control.
Suzuki, Takeshi; España, María Urizarna; Nunes, Maria Andreia; Zhurov, Vladimir; Dermauw, Wannes; Osakabe, Masahiro; Leeuwen, Thomas V. Van; Grbic, Miodrag; Grbic, Vojislava
Protocols for the delivery of small molecules to the two-spotted spider mite, Tetranychus urticae Journal Article
In: PLOS ONE, vol. 12, iss. 7, pp. e0180658, 2017, ISSN: 1932-6203.
Abstract | Links | BibTeX | Tags: Diet, Eggs, Fluorescent dyes, Larvae, Leaves, Mites, Small molecules, Tracer dye staining
@article{Suzuki2017,
title = {Protocols for the delivery of small molecules to the two-spotted spider mite, Tetranychus urticae},
author = {Takeshi Suzuki and María Urizarna España and Maria Andreia Nunes and Vladimir Zhurov and Wannes Dermauw and Masahiro Osakabe and Thomas V. Van Leeuwen and Miodrag Grbic and Vojislava Grbic},
url = {https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0180658},
doi = {10.1371/JOURNAL.PONE.0180658},
issn = {1932-6203},
year = {2017},
date = {2017-01-01},
journal = {PLOS ONE},
volume = {12},
issue = {7},
pages = {e0180658},
publisher = {Public Library of Science},
abstract = {The two-spotted spider mite, Tetranychus urticae, is a chelicerate herbivore with an extremely wide host range and an extraordinary ability to develop pesticide resistance. Due to its responsiveness to natural and synthetic xenobiotics, the spider mite is becoming a prime pest herbivore model for studies of the evolution of host range, plant-herbivore interactions and mechanisms of xenobiotic resistance. The spider mite genome has been sequenced and its transcriptional responses to developmental and various biotic and abiotic cues have been documented. However, to identify biological and evolutionary roles of T. urticae genes and proteins, it is necessary to develop methods for the efficient manipulation of mite gene function or protein activity. Here, we describe protocols developed for the delivery of small molecules into spider mites. Starting with mite maintenance and the preparation of the experimental mite populations of developmentally synchronized larvae and adults, we describe 3 methods for delivery of small molecules including artificial diet, leaf coating, and soaking. The presented results define critical steps in these methods and demonstrate that they can successfully deliver tracer dyes into mites. Described protocols provide guidelines for high-throughput setups for delivery of experimental compounds that could be used in reverse genetics platforms to modulate gene expression or protein activity, or for screens focused on discovery of new molecules for mite control. In addition, described protocols could be adapted for other Tetranychidae and related species of economic importance such as Varroa, dust and poultry mites.},
keywords = {Diet, Eggs, Fluorescent dyes, Larvae, Leaves, Mites, Small molecules, Tracer dye staining},
pubstate = {published},
tppubtype = {article}
}
The two-spotted spider mite, Tetranychus urticae, is a chelicerate herbivore with an extremely wide host range and an extraordinary ability to develop pesticide resistance. Due to its responsiveness to natural and synthetic xenobiotics, the spider mite is becoming a prime pest herbivore model for studies of the evolution of host range, plant-herbivore interactions and mechanisms of xenobiotic resistance. The spider mite genome has been sequenced and its transcriptional responses to developmental and various biotic and abiotic cues have been documented. However, to identify biological and evolutionary roles of T. urticae genes and proteins, it is necessary to develop methods for the efficient manipulation of mite gene function or protein activity. Here, we describe protocols developed for the delivery of small molecules into spider mites. Starting with mite maintenance and the preparation of the experimental mite populations of developmentally synchronized larvae and adults, we describe 3 methods for delivery of small molecules including artificial diet, leaf coating, and soaking. The presented results define critical steps in these methods and demonstrate that they can successfully deliver tracer dyes into mites. Described protocols provide guidelines for high-throughput setups for delivery of experimental compounds that could be used in reverse genetics platforms to modulate gene expression or protein activity, or for screens focused on discovery of new molecules for mite control. In addition, described protocols could be adapted for other Tetranychidae and related species of economic importance such as Varroa, dust and poultry mites.