Publications

@article{Mendes2022,

title = {Antibacterial action and target mechanisms of zinc oxide nanoparticles against bacterial pathogens},

author = {Carolina Rosai Mendes and Guilherme Dilarri and Carolina Froes Forsan and Vinícius Moraes Ruy Sapata and Paulo Renato Matos Lopes and Peterson Bueno Moraes and Renato Nallin Montagnolli and Henrique Ferreira and Ederio Dino Bidoia},

url = {https://www.nature.com/articles/s41598-022-06657-y},

doi = {10.1038/s41598-022-06657-y},

issn = {2045-2322},

year  = {2022},

date = {2022-01-01},

journal = {Scientific Reports 2022 12:1},

volume = {12},

issue = {1},

pages = {1-10},

publisher = {Nature Publishing Group},

abstract = {Zinc oxide nanoparticles (ZnO NPs) are one of the most widely used nanoparticulate materials due to their antimicrobial properties, but their main mechanism of action (MOA) has not been fully elucidated. This study characterized ZnO NPs by using X-ray diffraction, FT-IR spectroscopy and scanning electron microscopy. Antimicrobial activity of ZnO NPs against the clinically relevant bacteria Escherichia coli, Staphylococcus aureus, Pseudomonas aeruginosa, and the Gram-positive model Bacillus subtilis was evaluated by performing resazurin microtiter assay (REMA) after exposure to the ZnO NPs at concentrations ranging from 0.2 to 1.4 mM. Sensitivity was observed at 0.6 mM for the Gram-negative and 1.0 mM for the Gram-positive cells. Fluorescence microscopy was used to examine the interference of ZnO NPs on the membrane and the cell division apparatus of B. subtilis (amy::pspac-ftsZ-gfpmut1) expressing FtsZ-GFP. The results showed that ZnO NPs did not interfere with the assembly of the divisional Z-ring. However, 70% of the cells exhibited damage in the cytoplasmic membrane after 15 min of exposure to the ZnO NPs. Electrostatic forces, production of Zn2+ ions and the generation of reactive oxygen species were described as possible pathways of the bactericidal action of ZnO. Therefore, understanding the bactericidal MOA of ZnO NPs can potentially help in the construction of predictive models to fight bacterial resistance.},

keywords = {Applied Microbiology, Bacteria, Nanoparticles},

pubstate = {published},

tppubtype = {article}

}

@article{Savi2020,

title = {Dihydroisocoumarins produced by Diaporthe cf. heveae LGMF1631 inhibiting citrus pathogens},

author = {Daiani Cristina Savi and Sandriele Aparecida Noriler and Larissa V. Ponomareva and Jon S. Thorson and Jürgen Rohr and Chirlei Glienke and Khaled A. Shaaban},

url = {https://link.springer.com/article/10.1007/s12223-019-00746-8},

doi = {10.1007/S12223-019-00746-8/METRICS},

issn = {18749356},

year  = {2020},

date = {2020-01-01},

journal = {Folia Microbiologica},

volume = {65},

issue = {2},

pages = {381-392},

publisher = {Springer},

abstract = {Citrus black spot (CBS) and post-bloom fruit drop (PFD), caused by Phyllosticta citricarpa and Colletotrichum abscissum, respectively, are two important citrus diseases worldwide. CBS depreciates the market value and prevents exportation of citrus fruits to Europe. PFD under favorable climatic conditions can cause the abscission of flowers, thereby reducing citrus production by 80%. An ecofriendly alternative to control plant diseases is the use of endophytic microorganisms, or secondary metabolites produced by them. Strain LGMF1631, close related to Diaporthe cf. heveae 1, was isolated from the medicinal plant Stryphnodendron adstringens and showed significant antimicrobial activity, in a previous study. In view of the potential presented by strain LGMF1631, and the absence of chemical data for secondary metabolites produced by D. cf. heveae, we decided to characterize the compounds produced by strain LGMF1631. Based on ITS, TEF1, and TUB phylogenetic analysis, strain LGMF1631 was confirmed to belong to D. cf. heveae 1. Chemical assessment of the fungal strain LGMF1631 revealed one new seco-dihydroisocoumarin [cladosporin B (1)] along with six other related, already known dihydroisocoumarin derivatives and one monoterpene [(−)-(1S,2R,3S,4R)-p-menthane-1,2,3-triol (8)]. Among the isolated metabolites, compound 5 drastically reduced the growth of both phytopathogens in vitro and completely inhibited the development of CBS and PFD in citrus fruits and flowers. In addition, compound 5 did not show toxicity against human cancer cell lines or citrus leaves, at concentrations higher than used for the inhibition of the phytopathogens, suggesting the potential use of (−)-(3R,4R)-cis-4-hydroxy-5-methylmellein (5) to control citrus diseases.},

keywords = {Applied Microbiology, Environmental Engineering/Biotechnology, general, Immunology, Life Sciences, Microbiology},

pubstate = {published},

tppubtype = {article}

}

@article{Savi2019,

title = {Secondary metabolites produced by Microbacterium sp. LGMB471 with antifungal activity against the phytopathogen Phyllosticta citricarpa},

author = {Daiani Cristina Savi and Jürgen Rohr and Khaled A. Shaaban and Francielly M. W. Gos and Jon S. Thorson and Chirlei Glienke},

url = {https://link.springer.com/article/10.1007/s12223-018-00668-x},

doi = {10.1007/S12223-018-00668-X/METRICS},

issn = {18749356},

year  = {2019},

date = {2019-01-01},

journal = {Folia Microbiologica},

volume = {64},

issue = {3},

pages = {453-460},

publisher = {Springer Science and Business Media B.V.},

abstract = {The citrus black spot (CBS), caused by Phyllosticta citricarpa, is one of the most important citrus diseases in subtropical regions of Africa, Asia, Oceania, and the Americas, and fruits with CBS lesions are still subject to quarantine regulations in the European Union. Despite the high application of fungicides, the disease remains present in the citrus crops of Central and South America. In order to find alternatives to help control CBS and reduce the use of fungicides, we explored the antifungal potential of endophytic actinomycetes isolated from the Brazilian medicinal plant Vochysia divergens found in the Pantanal biome. Two different culture media and temperatures were selected to identify the most efficient conditions for the production of active secondary metabolites. The metabolites produced by strain Microbacterium sp. LGMB471 cultured in SG medium at 36 °C considerably inhibited the development of P. citricarpa. Three isoflavones and five diketopiperazines were identified, and the compounds 7-O-β-D-glucosyl-genistein and 7-O-β-D-glucosyl-daidzein showed high activity against P. citricarpa, with the MIC of 33 μg/mL and inhibited the production of asexual spores of P. citricarpa on leaves and citrus fruits. Compounds that inhibit conidia formation may be a promising alternative to reduce the use of fungicides in the control of CBS lesions, especially in regions where sexual reproduction does not occur, as in the USA. Our data suggest the use of Microbacterium sp. LGMB471 or its metabolites as an ecological alternative to be used in association with the fungicides for the control of CBS disease.},

keywords = {Applied Microbiology, Environmental Engineering/Biotechnology, general, Immunology, Life Sciences, Microbiology},

pubstate = {published},

tppubtype = {article}

}

@article{nokey,

title = {Bioprospecting of Diaporthe terebinthifolii LGMF907 for antimicrobial compounds},

author = {Aliandra G. Medeiros and Daiani C. Savi and Prithiba Mitra and Khaled A. Shaaban and Amit K. Jha and Jon S. Thorson and Jürgen Rohr and Chirlei Glienke},

url = {https://link.springer.com/article/10.1007/s12223-018-0587-2},

doi = {10.1007/S12223-018-0587-2},

issn = {1874-9356},

year  = {2018},

date = {2018-01-01},

journal = {Folia Microbiologica 2018 63:4},

volume = {63},

issue = {4},

pages = {499-505},

publisher = {Springer},

abstract = {Antibiotic-resistant bacteria have been observed with increasing frequency over the past decades, driving the search for new drugs and stimulating the interest in natural products sources. Endophytic fungi from medicinal plants represent a great source of novel bioactive compounds useful to pharmaceutical and agronomical purposes. Diaporthe terebinthifolii is an endophytic species isolated from Schinus terebinthifolius, a plant used in popular medicine for several health problems. The strain D. terebinthifolii LGMF907 was previously reported by our group to produce secondary metabolites with biological activity against phytopathogens. Based on these data, strain LGMF907 was chosen for bioprospecting against microorganisms of clinical importance and for characterization of major secondary metabolites. In this study, different culture conditions were evaluated and the biological activity of this strain was expanded. The crude extracts demonstrated high antibacterial activity against Escherichia coli, Micrococcus luteus, Saccharomyces cerevisiae, methicillin-sensitive Staphylococcus aureus, and methicillin-resistant S. aureus. The compounds diaporthin and orthosporin were characterized and also showed activity against the clinical microorganisms evaluated. This study discloses the first isolation of diaporthin and orthosporin from D. terebinthifolii, and revealed the potential of this endophytic fungus to produce secondary metabolites with antimicrobial activity.},

keywords = {Applied Microbiology, Environmental Engineering/Biotechnology, general, Immunology, Life Sciences, Microbiology},

pubstate = {published},

tppubtype = {article}

}

@article{nokey,

title = {Bioprospecting of Diaporthe terebinthifolii LGMF907 for antimicrobial compounds},

author = {Aliandra G. Medeiros and Daiani C. Savi and Prithiba Mitra and Khaled A. Shaaban and Amit K. Jha and Jon S. Thorson and Jürgen Rohr and Chirlei Glienke},

url = {https://link.springer.com/article/10.1007/s12223-018-0587-2},

doi = {10.1007/S12223-018-0587-2},

issn = {1874-9356},

year  = {2018},

date = {2018-01-01},

journal = {Folia Microbiologica 2018 63:4},

volume = {63},

issue = {4},

pages = {499-505},

publisher = {Springer},

abstract = {Antibiotic-resistant bacteria have been observed with increasing frequency over the past decades, driving the search for new drugs and stimulating the interest in natural products sources. Endophytic fungi from medicinal plants represent a great source of novel bioactive compounds useful to pharmaceutical and agronomical purposes. Diaporthe terebinthifolii is an endophytic species isolated from Schinus terebinthifolius, a plant used in popular medicine for several health problems. The strain D. terebinthifolii LGMF907 was previously reported by our group to produce secondary metabolites with biological activity against phytopathogens. Based on these data, strain LGMF907 was chosen for bioprospecting against microorganisms of clinical importance and for characterization of major secondary metabolites. In this study, different culture conditions were evaluated and the biological activity of this strain was expanded. The crude extracts demonstrated high antibacterial activity against Escherichia coli, Micrococcus luteus, Saccharomyces cerevisiae, methicillin-sensitive Staphylococcus aureus, and methicillin-resistant S. aureus. The compounds diaporthin and orthosporin were characterized and also showed activity against the clinical microorganisms evaluated. This study discloses the first isolation of diaporthin and orthosporin from D. terebinthifolii, and revealed the potential of this endophytic fungus to produce secondary metabolites with antimicrobial activity.},

keywords = {Applied Microbiology, Environmental Engineering/Biotechnology, general, Immunology, Life Sciences, Microbiology},

pubstate = {published},

tppubtype = {article}

}

@article{nokey,

title = {Bioprospecting of Diaporthe terebinthifolii LGMF907 for antimicrobial compounds},

author = {Aliandra G. Medeiros and Daiani C. Savi and Prithiba Mitra and Khaled A. Shaaban and Amit K. Jha and Jon S. Thorson and Jürgen Rohr and Chirlei Glienke},

url = {https://link.springer.com/article/10.1007/s12223-018-0587-2},

doi = {10.1007/S12223-018-0587-2},

issn = {1874-9356},

year  = {2018},

date = {2018-01-01},

journal = {Folia Microbiologica 2018 63:4},

volume = {63},

issue = {4},

pages = {499-505},

publisher = {Springer},

abstract = {Antibiotic-resistant bacteria have been observed with increasing frequency over the past decades, driving the search for new drugs and stimulating the interest in natural products sources. Endophytic fungi from medicinal plants represent a great source of novel bioactive compounds useful to pharmaceutical and agronomical purposes. Diaporthe terebinthifolii is an endophytic species isolated from Schinus terebinthifolius, a plant used in popular medicine for several health problems. The strain D. terebinthifolii LGMF907 was previously reported by our group to produce secondary metabolites with biological activity against phytopathogens. Based on these data, strain LGMF907 was chosen for bioprospecting against microorganisms of clinical importance and for characterization of major secondary metabolites. In this study, different culture conditions were evaluated and the biological activity of this strain was expanded. The crude extracts demonstrated high antibacterial activity against Escherichia coli, Micrococcus luteus, Saccharomyces cerevisiae, methicillin-sensitive Staphylococcus aureus, and methicillin-resistant S. aureus. The compounds diaporthin and orthosporin were characterized and also showed activity against the clinical microorganisms evaluated. This study discloses the first isolation of diaporthin and orthosporin from D. terebinthifolii, and revealed the potential of this endophytic fungus to produce secondary metabolites with antimicrobial activity.},

keywords = {Applied Microbiology, Environmental Engineering/Biotechnology, general, Immunology, Life Sciences, Microbiology},

pubstate = {published},

tppubtype = {article}

}