Publicações

@article{Merfa2022,

title = {Probing the Application of OmpA-Derived Peptides to Disrupt the Acquisition of ‘Candidatus Liberibacter asiaticus’ by Diaphorina citri},

author = {Marcus Vinıcius Merfa and Eduarda Regina Fischer and Mariana Silva and Carolina Sardinha Francisco and Helvecio Della Coletta-Filho and Alessandra Alves Souza},

url = {https://apsjournals.apsnet.org/doi/10.1094/PHYTO-06-21-0252-FI},

doi = {10.1094/PHYTO-06-21-0252-FI/ASSET/IMAGES/LARGE/PHYTO-06-21-0252-FIF6.JPEG},

issn = {19437684},

year  = {2022},

date = {2022-01-01},

journal = {Phytopathology},

volume = {112},

issue = {1},

pages = {163-172},

publisher = {American Phytopathological Society},

abstract = {Huanglongbing (HLB) is currently the most devastating disease of citrus worldwide. Both bacteria ‘Candidatus Liberibacter asiaticus’ (CLas) and ‘Candidatus Liberibacter americanus’ (CLam) are associated with HLB in Brazil but with a strong prevalence of CLas over CLam. Conventionally, HLB management focuses on controlling the insect vector population (Diaphorina citri; also known as Asian citrus psyllid [ACP]) by spraying insecticides, an approach demonstrated to be mostly ineffective. Thus, development of novel, more efficient HLB control strategies is required. The multifunctional bacterial outer membrane protein OmpA is involved in several molecular processes between bacteria and their hosts and has been suggested as a target for bacterial control. Curiously, OmpA is absent in CLam in comparison with CLas, suggesting a possible role in host interaction. Therefore, in the current study, we have treated ACPs with different OmpA-derived peptides, aiming to evaluate acquisition of CLas by the insect vector. Treatment of psyllids with 5 µM of Pep1, Pep3, Pep5, and Pep6 in artificial diet significantly reduced the acquisition of CLas, whereas increasing the concentration of Pep5 and Pep6 to 50 µM abolished this process. In addition, in planta treatment with 50 µM of Pep6 also significantly decreased the acquisition of CLas, and sweet orange plants stably absorbed and maintained this peptide for as long as 3 months post the final application. Together, our results demonstrate the promising use of OmpA-derived peptides as a novel biotechnological tool to control CLas.},

keywords = {Bacterial pathogens, Biotechnology, Disease control and pest management, genetics},

pubstate = {published},

tppubtype = {article}

}

@article{Mendes2022,

title = {Antibacterial action and target mechanisms of zinc oxide nanoparticles against bacterial pathogens},

author = {Carolina Rosai Mendes and Guilherme Dilarri and Carolina Froes Forsan and Vinícius Moraes Ruy Sapata and Paulo Renato Matos Lopes and Peterson Bueno Moraes and Renato Nallin Montagnolli and Henrique Ferreira and Ederio Dino Bidoia},

url = {https://www.nature.com/articles/s41598-022-06657-y},

doi = {10.1038/s41598-022-06657-y},

issn = {2045-2322},

year  = {2022},

date = {2022-01-01},

journal = {Scientific Reports 2022 12:1},

volume = {12},

issue = {1},

pages = {1-10},

publisher = {Nature Publishing Group},

abstract = {Zinc oxide nanoparticles (ZnO NPs) are one of the most widely used nanoparticulate materials due to their antimicrobial properties, but their main mechanism of action (MOA) has not been fully elucidated. This study characterized ZnO NPs by using X-ray diffraction, FT-IR spectroscopy and scanning electron microscopy. Antimicrobial activity of ZnO NPs against the clinically relevant bacteria Escherichia coli, Staphylococcus aureus, Pseudomonas aeruginosa, and the Gram-positive model Bacillus subtilis was evaluated by performing resazurin microtiter assay (REMA) after exposure to the ZnO NPs at concentrations ranging from 0.2 to 1.4 mM. Sensitivity was observed at 0.6 mM for the Gram-negative and 1.0 mM for the Gram-positive cells. Fluorescence microscopy was used to examine the interference of ZnO NPs on the membrane and the cell division apparatus of B. subtilis (amy::pspac-ftsZ-gfpmut1) expressing FtsZ-GFP. The results showed that ZnO NPs did not interfere with the assembly of the divisional Z-ring. However, 70% of the cells exhibited damage in the cytoplasmic membrane after 15 min of exposure to the ZnO NPs. Electrostatic forces, production of Zn2+ ions and the generation of reactive oxygen species were described as possible pathways of the bactericidal action of ZnO. Therefore, understanding the bactericidal MOA of ZnO NPs can potentially help in the construction of predictive models to fight bacterial resistance.},

keywords = {Applied Microbiology, Bacteria, Nanoparticles},

pubstate = {published},

tppubtype = {article}

}

@article{Martins2022,

title = {Initial productive performance of ‘Tahiti’ acid lime considering different rootstocks and levels of salt water},

author = {Gabriel Oliveira Martins and Tainá Alves Silva and Alesson Souza Silva and Larissa Lorrane Santos and Damares Francisco Correia Nascimento and Pedro Dantas Fernandes and Walter Santos Soares Filho and Marcos Eric Barbosa Brito},

url = {http://www.scielo.br/j/rca/a/Yr4JFjqSXFYMKYvvkrPWmWM/?lang=en},

doi = {10.5935/1806-6690.20220019},

issn = {0045-6888},

year  = {2022},

date = {2022-01-01},

journal = {Revista Ciência Agronômica},

volume = {53},

issue = {9},

pages = {2022},

publisher = {Universidade Federal do Ceará},

abstract = {ABSTRACT The productive performance of citrus plants is limited by abiotic factors such as salinity. The response to these factors varies among rootstocks, and to guarantee sustainability of the production system, it is possible to identify the most suitable genotypes agronomically for each growing environment. Given the above, the aim of this study was to evaluate the productive performance of the ‘Tahiti’ acid lime combined with different citrus rootstocks irrigated with water at different levels of salinity during the first year of fruit production. Three levels of electrical conductivity of the irrigation water (0.14, 2.40 and 4.80 dS m-1) and 13 rootstocks (‘Santa Cruz Rangpur’ lime, ‘Indio’, ‘Riverside’ and ‘San Diego’ citrandarins, ‘Sunki Tropical’ mandarin and eight hybrids generated by the Citrus Breeding Program of Embrapa Cassava & Fruits). The experimental design was of randomised blocks, with four replications and one plant per plot. The fruits were harvested from 300 to 720 days after transplanting (DAT) to pots with a capacity of 60 L, which had been adapted for use as lysimeters. Fruit production was determined by weight and number, in addition to measuring the size and the thickness of the peel. It was found that water of up to 2.4 dS m-1 can be used for citrus irrigation without significantly compromising early fruit production in the ‘Tahiti’ acid lime when grafted on the ‘Sunki Tropical’ mandarin, ‘Riverside’ citrandarin, and on the hybrids TSKC x TRBK - 007, TSKC x CTTR - 012, HTR - 069, TSKC x (LCR x TR) - 040 and TSKC x (LCR x TR) - 059, which resulted in an earlier start to production. At the salinity level of 4.8 dS m-1, the ‘Tahiti’ acid lime proved to be more productive and less sensitive when grafted onto ‘Sunki Tropical’ mandarin, TSKC x TRBK - 007, TSKC x CTTR - 012, HTR - 069, and TSKC x (LCR x TR) - 040 hybrids.},

keywords = {Citrus ×latifolia, Citrus spp, Poncirus hybrids, Salt stress},

pubstate = {published},

tppubtype = {article}

}

@article{Martinelli2022,

title = {Glyphosate Excessive Use Affects Citrus Growth and Yield: The Vicious (and Unsustainable) Circle in Brazilian Orchards},

author = {Rodrigo Martinelli and Luiz Renato Rufino and Ricardo Alcántara-De Cruz and Patricia Marluci Conceição and Patricia Andrea Monquero and Fernando Alves Azevedo},

url = {https://www.mdpi.com/2073-4395/12/2/453/htm https://www.mdpi.com/2073-4395/12/2/453},

doi = {10.3390/AGRONOMY12020453/S1},

issn = {20734395},

year  = {2022},

date = {2022-01-01},

journal = {Agronomy},

volume = {12},

issue = {2},

pages = {453},

publisher = {MDPI},

abstract = {The excessive use of glyphosate by Brazilian citrus growers leads to a vicious and unsustainable circle: Increasing the glyphosate use and the selection pressure of resistant/tolerant weeds, as well as the phytointoxication of the crop. In addition, there is speculation on the consequences of using glyphosate and the studies are not conclusive. Therefore, this study aimed to evaluate the glyphosate management in citrus orchards by assessing its effects in a 5-year field experiment using different doses and application frequencies. Here, we determine the weed control levels, the orchard growth and fruit yield, as well as the economic viability of the treatments. Higher weed control was observed more often with the increasing frequency of glyphosate application, and occasionally with increasing doses. However, some species predominated even at high glyphosate usage, such as BIDPI (Bidens pilosa (L.)), RAPRA (Raphanus raphanistrum (L.)), and ERICA (Conyza canadensis (L.) Cronquist). Phytotoxicity symptoms were demonstrated up to the fourth year of the orchard, and onwards the plants no longer expressed them. This was a highlight, since there was a decrease over time in growth (up to 5.3 m3) and fruit yield (up to 36.3 t ha−1), with losses that reached −56% of the total income. This is the first report to demonstrate that the increase in glyphosate usage can occasionally increase weed control, but it can also decrease orchard development and its financial via-bility.},

keywords = {Herbicide drift, phytotoxicity, Return of in-vestment, Weed control, Weed resistance, Yield drop},

pubstate = {published},

tppubtype = {article}

}

@article{Junior2022,

title = {Performance of citrumelos in area affected by citrus sudden death},

author = {Jorgino Pompeu Junior and Silvia Blumer and Josiane Rodrigues and Eduardo Sanches Stuchi and Eduardo Augusto Girardi},

url = {https://rsdjournal.org/index.php/rsd/article/view/24527},

doi = {10.33448/RSD-V11I1.24527},

issn = {2525-3409},

year  = {2022},

date = {2022-01-01},

journal = {Research, Society and Development},

volume = {11},

issue = {1},

pages = {e25411124527-e25411124527},

publisher = {Research, Society and Development},

abstract = {Citrus sudden death (CSD) is a biotic disease of unknown origin, transmitted by aerial vectors and buds that affects sweet oranges and tangerines cultivars budded on Rangpur lime, and Volkamer and Rough lemons rootstocks. The same cultivars onto Cleopatra and Sunki mandarins, trifoliate orange and Swingle citrumelo are asymptomatic. The objective of this study was to evaluate nine selections of citrumelo regarding tolerance to CSD and their effects on growth and production of Valencia sweet orange trees cultivated without irrigation. A completely randomized design with nine treatments (citrumelos) and five replicates, each plot represented by one tree. After 16 years in the field, no tree showed symptoms of CSD or bud union ring between the scion and the rootstock. After 14 yields, the trees onto Swingle, W-2, F80-7 and F80-8 yielded the highest productions and W-2 and F80-7 induced yearly production and did not show alternate bearing. The W-2, Swingle and F80-7 citrumelos induced the largest trees and the F80-5, F80-3 and F81-18 the smaller trees. Although they do not induce high fruit production per tree, they have the highest production efficiencies, and F80-5 motivated early production and a smaller canopy diameter, enabling greater planting density.},

keywords = {Disease, Poncirus trifoliata, rootstock, Trifoliate orange hybrid.},

pubstate = {published},

tppubtype = {article}

}

@article{Goulin2022,

title = {Genome Sequence Resources of Colletotrichum abscissum, the Causal Agent of Citrus Post-Bloom Fruit Drop, and the Closely Related Species C. filicis},

author = {Eduardo Goulin and Thais Regina Boufleur and Francesca Negrini and Greice Amaral Carneiro and Elena Baraldi and Marcos Antonio Machado and Gaetan Le Floch and Riccardo Baroncelli},

url = {https://apsjournals.apsnet.org/doi/10.1094/PHYTO-05-22-0176-A},

doi = {10.1094/PHYTO-05-22-0176-A},

issn = {0031-949X},

year  = {2022},

date = {2022-01-01},

journal = {https://doi.org/10.1094/PHYTO-05-22-0176-A},

volume = {260},

pages = {126938},

publisher = {The American Phytopathological Society},

abstract = {The citrus crop is one of the most important culture worldwide, being Brazil the world highest producer of sweet oranges. The challenge for this culture is the constant attack by several pathogens that cause economically losses. Among the fungal diseases the post-bloom fruit drop causes dramatically losses in production, characterized by damages in the blossoms that causes fruit infeasibility and early drop, one of the causal agent is Colletotrichum abscissum. The pathogen control is based in chemical applications, irrespective of crops and fungicides used, development of fungicide-resistant pathogen populations is a problem in agriculture. New technologies are being applied every year to better understand the pathogens biology, which can contribute to plant diseases control as an alternative to chemical compounds. The RNA interference emerged as a potential technology for gene function studies as well as an approach for pathogens control. The fungicides compounds have action in different targets, such as the succinate dehydrogenase (SDH), which plays a hole in cell respiration. Therefore, here we investigate the functionality of the RNAi machinery of C. abscissum and test genetically whether the chemically pre-defined fungal SDH target may represent a promising target gene in RNAi based control strategies. The C. abscissum RNAi machinery was functionally proven by silencing of gene report. Then, the silencing of SDH subunits were induced and verified. In addition, the C. abscissum mutants generated for this study made possible the fungus infection process investigation. Furthermore, knockdown mutants of succinate dehydrogenase subunits genes resulted in morphological and significant pathogenicity changes. Thus, in conclusion, we suggest that the RNA interference is an important tool that can be exploited to post bloom fruit drop disease control and also the chemical fungicide target are still useful in the new technologies control strategies.},

keywords = {genomics, plant-pathogenic fungus, post-bloom fruit drop},

pubstate = {published},

tppubtype = {article}

}

@article{Fujimoto2022,

title = {Biocontrol of Phyllosticta citricarpa by Bacillus spp.: biological and chemical aspects of the microbial interaction},

author = {Andréia Fujimoto and Fábio Augusto and Taicia Pacheco Fill and Rafael Kupper Moretto and Katia Cristina Kupper},

url = {https://link.springer.com/article/10.1007/s11274-021-03214-z},

doi = {10.1007/S11274-021-03214-Z/METRICS},

issn = {15730972},

year  = {2022},

date = {2022-01-01},

journal = {World Journal of Microbiology and Biotechnology},

volume = {38},

issue = {3},

pages = {1-14},

publisher = {Springer Science and Business Media B.V.},

abstract = {Citrus fruits are the most produced fruits in the world, but they are threatened by several pathogens, including the fungus Phyllosticta citricarpa, the causal agent of citrus black spot (CBS). The fungus affects most citrus species and the infection results in economic losses in citrus-producing areas. This disease causes the aesthetic depreciation of fresh fruit, impairing its commercialization. As an alternative to the use of synthetic fungicides to control the pathogen, the biological control, using bacteria of the genus Bacillus, is highlighted. Such microorganisms enable biocontrol by the production of volatile organic compounds (VOC) or non-volatile. Therefore, this work aimed to evaluate the production of VOC by isolates of Bacillus spp. grown in different culture media; to evaluate the effects of these compounds on the evolution of CBS lesions in orange fruits; to study the effects of VOC on resistance induction in orange fruits; to evaluate the effects of VOC on P. citricarpa morphology in CBS lesions, and to identify the produced VOC. Tryptone soya agar (TSA) and tryptone soya broth (TSB) media used to culture the bacterium resulted in up to 73% pathogen inhibition by VOC. Volatile compounds from Bacillus spp. ACB-65 and Bacillus spp. ACB-73 when cultured in TSB culture medium provided 86% inhibition of freckles that evolved to hard spots. The volatile fractions produced by the bacteria were identified as alcohols, ketones, amines, ethers, aldehydes and carboxylic acids that can serve as arsenal against the phytopathogen. The present work demonstrated the potential of VOC produced by Bacillus spp. in the control of P. citricarpa.},

keywords = {biological control, Citrus sinensis, Enzymatic activities, Guignardia citricarpa, Volatile organic compounds},

pubstate = {published},

tppubtype = {article}

}

@article{nokey,

title = {An Extracytoplasmic Function Sigma Factor Required for Full Virulence in Xanthomonas citri pv. citri},

author = {Lídia Passos Lima and Juliana Biar Pereira and Anthony Jhoao Fasabi Flores and Alan Péricles Rodrigues Lorenzetti and Ana Laura Boechat and Maria Claudia Pereda and Sophia Gualtieri and Daniele Ferreira Prado and Diego Rocha and Lucas Moraes Ceseti and Regina Lúcia Baldini and Chuck S. Farah and Tie Koide and Celso Eduardo Benedetti and Cristina E. Alvarez-Martinez},

url = {https://journals.asm.org/doi/10.1128/jb.00624-21},

doi = {10.1128/JB.00624-21/SUPPL_FILE/JB.00624-21-S0002.XLSX},

issn = {10985530},

year  = {2022},

date = {2022-01-01},

journal = {Journal of Bacteriology},

volume = {204},

issue = {5},

publisher = {American Society for Microbiology},

abstract = {The genus Xanthomonas includes more than 30 phytopathogenic species that infect a wide range of plants and cause severe diseases that greatly impact crop productivity. These bacteria are highly adapted to the soil and plant environment, being found in decaying material, as epiphytes, and colonizing the plant mesophyll. Signal transduction mechanisms involved in the responses of Xanthomonas to environmental changes are still poorly characterized. Xanthomonad genomes typically encode several representatives of the extracytoplasmic function s (s ECF) factors, whose physiological roles remain elusive. In this work, we functionally characterized the Xanthomonas citri pv. citri EcfL, a s ECF factor homologous to members of the iron-responsive FecI-like group. We show that EcfL is not required or induced during iron starvation, despite presenting the common features of other FecI-like s ECF factors. EcfL positively regulates one operon composed of three genes that encode a TonB-dependent receptor involved in cell surface signaling, an acid phosphatase, and a lectin-domain containing protein. Furthermore, we demonstrate that EcfL is required for full virulence in citrus, and its regulon is induced inside the plant mesophyll and in response to acid stress. Together, our study suggests a role for EcfL in the adaptation of X. citri to the plant environment, in this way contributing to its ability to cause citrus canker disease.},

keywords = {phytopathogens, regulation of gene expression, sigma factors},

pubstate = {published},

tppubtype = {article}

}

@article{Dilarri2022,

title = {Evaluation of calcium hydroxide, calcium hypochlorite, peracetic acid, and potassium bicarbonate as citrus fruit sanitizers},

author = {Guilherme Dilarri and Caio Felipe Cavicchia Zamuner and Mauricio Bacci and Henrique Ferreira},

url = {https://link.springer.com/article/10.1007/s13197-021-05185-3},

doi = {10.1007/S13197-021-05185-3/METRICS},

issn = {09758402},

year  = {2022},

date = {2022-01-01},

journal = {Journal of Food Science and Technology},

volume = {59},

issue = {5},

pages = {1739-1747},

publisher = {Springer},

abstract = {Xanthomonas citri (X. citri) is a quarentenary plant pathogen and the causal agent of the citrus canker. X. citri forms biofilms and remains fixed on the surface of plant tissues, especially on leaves and fruits. Considering this, all the citrus fruits have to be sanitized before they can be commercialized. NaOCl is the main sanitizer used to decontaminate fruits in the world. Due to its toxicity, treatment with NaOCl is no longer accepted by some Europe Union countries. Therefore, the aim of this work was to evaluate potassium bicarbonate (KHCO3), calcium hydroxide (Ca(OH)2), calcium hypochlorite (Ca(OCl)2) and peracetic acid (CH3CO3H) as alternatives to NaOCl for the sanitization of citrus fruit. By monitoring cell respiration and bacterial growth, we determined that peracetic acid and calcium hypochlorite exhibit bactericidal action against X. citri. Time-response growth curves and membrane integrity analyses showed that peracetic acid and calcium hypochlorite target the bacterial cytoplasmatic membrane, which is probably responsible for cell death in the first minutes of contact. The simulation of the sanitization process of citrus fruit in packinghouses showed that only peracetic acid exhibited a performance comparable to NaOCl. Among the tested compounds, peracetic acid constitutes an efficient and safer alternative to NaOCl.},

keywords = {Citrus canker, Orange, Packinghouse, Post-harvest sanitization, Sodium hypochlorite, Xanthomonas citri subsp. citri},

pubstate = {published},

tppubtype = {article}

}

@article{nokey,

title = {MqsR toxin as a biotechnological tool for plant pathogen bacterial control},

author = {Reinaldo Rodrigues Souza-Neto and Isis Gabriela Barbosa Carvalho and Paula Maria Moreira Martins and Simone Cristina Picchi and Juarez Pires Tomaz and Raquel Caserta and Marco Aurélio Takita and Alessandra Alves Souza},

url = {https://www.nature.com/articles/s41598-022-06690-x},

doi = {10.1038/s41598-022-06690-x},

issn = {2045-2322},

year  = {2022},

date = {2022-01-01},

journal = {Scientific Reports 2022 12:1},

volume = {12},

issue = {1},

pages = {1-12},

publisher = {Nature Publishing Group},

abstract = {Type II toxin-antitoxin (TA) systems are widespread in bacteria and are involved in important cell features, such as cell growth inhibition and antimicrobial tolerance, through the induction of persister cells. Overall, these characteristics are associated with bacterial survival under stress conditions and represent a significant genetic mechanism to be explored for antibacterial molecules. We verified that even though Xylella fastidiosa and Xanthomonas citri subsp. citri share closely related genomes, they have different Type II TA system contents. One important difference is the absence of mqsRA in X. citri. The toxin component of this TA system has been shown to inhibit the growth of X. fastidiosa. Thus, the absence of mqsRA in X. citri led us to explore the possibility of using the MqsR toxin to impair X. citri growth. We purified MqsR and confirmed that the toxin was able to inhibit X. citri. Subsequently, transgenic citrus plants producing MqsR showed a significant reduction in citrus canker and citrus variegated chlorosis symptoms caused, respectively, by X. citri and X. fastidiosa. This study demonstrates that the use of toxins from TA systems is a promising strategy to be explored aiming bacterial control.},

keywords = {Antimicrobials, Molecular engineering in plants},

pubstate = {published},

tppubtype = {article}

}

@article{nokey,

title = {Drought tolerance memory transmission by citrus buds},

author = {Andressa Rodrigues Oliveira Sousa and Rogério Ferreira Ribas and Mauricio Antônio Coelho Filho and Luciano Freschi and Claudia Fortes Ferreira and Walter Santos Soares Filho and Junior Pastor Pérez-Molina and Abelmon Silva Gesteira},

doi = {10.1016/J.PLANTSCI.2022.111292},

issn = {0168-9452},

year  = {2022},

date = {2022-01-01},

journal = {Plant Science},

volume = {320},

pages = {111292},

publisher = {Elsevier},

abstract = {Plants face recurrent drought events, and previous stresses can influence their responses to subsequent stress episodes. Studies on drought stress memory are recent in citriculture, although they show promise as a tool for crop improvement. Here, we investigated whether stress memory mechanisms can be detected in citrus plants grafted with buds from plants subjected to recurrent water deficit. Three rootstock varieties, namely ‘Rangpur Santa Cruz’ lime, ‘Sunki Maravilha’ mandarin and ‘Sunki Tropical’ mandarin, in combination with ‘Valencia’ orange, were either maintained under full irrigation or subjected to one, two, or three water deficit cycles. Buds from ‘Valencia’ orange were grafted onto ‘Swingle’ citrumelo rootstocks and were evaluated. This combination displayed improved physiological and biochemical performance under water limitation, especially ‘Valencia’ buds grafted onto ‘Sunki Maravilha’, with better photosynthetic performance under water deficit. These findings indicate that genotype-dependent epigenetic memory is a key factor in restoring citrus plants’ capacity to rely on previous stress experiences to restore better photosynthetic and physiological responses when undergoing new water deficit events. Therefore, epigenetic marks can be stored and transmitted to new citrus plants and are a promising alternative to enable increased water deficit tolerance when plants are then challenged by drought-prone environments.},

keywords = {Abiotic stress, Citrus, Epigenetics, Photosynthetic performance, water deficit},

pubstate = {published},

tppubtype = {article}

}

@article{nokey,

title = {Antibacterial Activity of Isobavachalcone (IBC) Is Associated with Membrane Disruption},

author = {Leticia Ribeiro Assis and Reinaldo Dos Santos Theodoro and Maria Beatriz Silva Costa and Julyanna Andrade Silva Nascentes and Miguel Divino Rocha and Meliza Arantes Souza Bessa and Ralciane Paula Menezes and Guilherme Dilarri and Giovane Böerner Hypolito and Vanessa Rodrigues Dos Santos and Cristiane Duque and Henrique Ferreira and Carlos Henrique Gomes Martins and Luis Octavio Regasini},

url = {https://www.mdpi.com/2077-0375/12/3/269/htm https://www.mdpi.com/2077-0375/12/3/269},

doi = {10.3390/MEMBRANES12030269/S1},

issn = {20770375},

year  = {2022},

date = {2022-01-01},

journal = {Membranes},

volume = {12},

issue = {3},

pages = {269},

publisher = {MDPI},

abstract = {Isobavachalcone (IBC) is a natural prenylated chalcone with a broad spectrum of pharmacological properties. In this work, we newly synthesized and investigated the antibacterial activity of IBC against Gram-positive, Gram-negative and mycobacterial species. IBC was active against Grampositive bacteria, mainly against Methicillin-Susceptible Staphylococcus aureus (MSSA) and MethicillinResistant Staphylococcus aureus (MRSA), with minimum inhibitory concentration (MIC) values of 1.56 and 3.12 µg/mL, respectively. On the other hand, IBC was not able to act against Gram-negative species (MIC > 400 µg/mL). IBC displayed activity against mycobacterial species (MIC = 64 µg/mL), including Mycobacterium tuberculosis, Mycobacterium avium and Mycobacterium kansasii. IBC was able to inhibit more than 50% of MSSA and MRSA biofilm formation at 0.78 µg/mL. Its antibiofilm activity was similar to vancomycin, which was active at 0.74 µg/mL. In order to study the mechanism of the action by fluorescence microscopy, the propidium iodide (PI) and SYTO9 fluorophores indicated that IBC disrupted the membrane of Bacillus subtilis. Toxicity assays using human keratinocytes (HaCaT cell line) showed that IBC did not have the capacity to reduce the cell viability. These results suggested that IBC is a promising antibacterial agent with an elucidated mode of action and potential applications as an antibacterial drug and a medical device coating.},

keywords = {Antibacterial, Biofilm, Chalcone, Membrane, Natural product},

pubstate = {published},

tppubtype = {article}

}

@article{nokey,

title = {Degree days, phenology and fruit quality of ‘Tahiti IAC-5’ acid lime grafted onto different rootstocks in Pindorama - SP},

author = {Bruna Aparecida Bettini-Tambur and Fernando Alves Azevedo and Mariângela Cristofani-Yaly and Maria Beatriz Bernardes Soares and Mônica Helena Martins and Evandro Henrique Schinor},

url = {http://www.scielo.br/j/rbf/a/p4vtqH6vMH78hHQzFfKpZtN/},

doi = {10.1590/0100-29452022064},

issn = {0100-2945},

year  = {2022},

date = {2022-01-01},

journal = {Revista Brasileira de Fruticultura},

volume = {44},

issue = {1},

publisher = {Sociedade Brasileira de Fruticultura},

abstract = {Abstract Few rootstock varieties are used for ‘Tahiti’ acid lime; therefore, diversification is necessary and studying the phenological phases of new scion/rootstock combinations is important for orchard management strategies. The aim of this study was to evaluate the influence of different rootstocks on the sum of degree days (DD) and on the phenology of plants and also on the quality of ‘Tahiti’ acid lime fruits, in Pindorama, SP. Six branches were marked on plants grafted onto 13 citrandarins (H), ‘Rangpur’ lime (RL), ‘Flying Dragon’ trifoliate orange (FD) and ‘Sunki’ mandarin (SM). Phenological characterization (anthesis to harvest) was performed using a grading scale. DD accumulation (anthesis to harvest) was determined considering the base temperature of 13 °C. Physical (weight, height and diameter, juice yield) and chemical (acidity, total soluble solids and ratio) characteristics of fruits were also evaluated. The experimental design was in randomized blocks with three replicates and one plant per plot. The rootstocks under study influenced DD accumulation and phenology, with early (H5, H73, H121, H135, H145, H152, FD - 1,558 at 1,676 DD and 135 to 146 days) and later combinations (H10, H14, H42, H47, H70, H150, H173, RL and SM - 1,694 to 1,829 DD and 148 to 157 days) regarding fruit maturation. The harvest point of ‘Tahiti’ acid lime fruits grafted onto H145 citrandarin and FD was advanced about 20 days in relation to the standard rootstock, RL. Total soluble solids content and fruit size were influenced by rootstocks; however, all scion/rootstock combinations presented fruits with quality characteristics within commercial standards.},

keywords = {‘Rangpur’ lime, citrandarin, Citrus × latifolia, Poncirus trifoliata ‘Flying Dragon},

pubstate = {published},

tppubtype = {article}

}

@article{Arena2022,

title = {A TaqMan RT-qPCR assay for absolute quantification of citrus leprosis virus C lineage SJP: disclosing the subgenomic/genomic ratio in plant and mite vector, plant organ-specific viral loads, and the kinetics of viral accumulation in plants},

author = {Gabriella D. Arena and Pedro L. Ramos-González and Aline D. Tassi and Marcos A. Machado and Juliana Freitas-Astúa},

url = {https://link.springer.com/article/10.1007/s40858-022-00539-4},

doi = {10.1007/S40858-022-00539-4/METRICS},

issn = {19832052},

year  = {2022},

date = {2022-01-01},

journal = {Tropical Plant Pathology},

pages = {1-12},

publisher = {Springer Science and Business Media LLC},

abstract = {Citrus leprosis virus C (CiLV-C) causes citrus leprosis, a re-emergent viral disease affecting citrus production in Latin America. Here, we developed two TaqMan RT-qPCR assays to detect and quantify CiLV-C lineage SJP, prevalent in the Brazilian citrus belt and the world’s main sweet orange production area. Assays targeted sequences within the genes p29 and RdRp. ORF p29 is transcribed in a subgenomic RNA (sgRNA) and codes for the putative capsid protein. ORF RdRp, coding for the replicase, is directly translated from the genomic RNA (gRNA). After assessing the efficiency and sensitivity of the assays, the targets were quantified in (i) symptomatic tissues of field-collected sweet orange (Citrus sinensis) samples, in a time course after infection in both (ii) Arabidopsis thaliana and (iii) sweet orange plants, and in (iv) the mite vector Brevipalpus yothersi. Sweet orange fruits support higher quantities of CiLV-C molecules than stems and leaves. Amounts of viral molecules in late lesions of different developmental stages collected in the field remain stable, but CiLV-C quantities progressively increase from the early stages of the infection to the appearance of symptoms in both A. thaliana and C. sinensis. The high sensibility of the assays allowed the quantification of CiLV-C in early infection periods, even during the asymptomatic period of plants, and in scant amounts of B. yothersi individuals. In plants, a higher accumulation of p29 than RdRp was reported (sgRNA/gRNA up to 95), reflecting the transcription of the p29 sgRNA. In mites, p29 quantities were only slightly higher than RdRp (sgRNA/gRNA of 2), adding a new tool to evaluate the putative replication of CiLV-C in its vector, a challenging aspect of the study of mite-virus interplay. The methods developed here contribute to a more accurate analysis of citrus leprosis epidemiology and shed light on unknown features of the virus-vector interaction.},

keywords = {Plant Pathology},

pubstate = {published},

tppubtype = {article}

}

@article{nokey,

title = {Syzygium aromaticum (clove) essential oil: An alternative for the sanitization of citrus fruit in packinghouses},

author = {Tiago Soraggi Battagin and Mario Nicolas Caccalano and Guilherme Dilarri and Caio Felipe Cavicchia Zamuner and Natália Alleoni and Luiz Leonardo Saldanha and Maurício Bacci and Henrique Ferreira},

url = {https://onlinelibrary.wiley.com/doi/full/10.1111/jfpp.15496 https://onlinelibrary.wiley.com/doi/abs/10.1111/jfpp.15496 https://ifst.onlinelibrary.wiley.com/doi/10.1111/jfpp.15496},

doi = {10.1111/JFPP.15496},

issn = {1745-4549},

year  = {2021},

date = {2021-01-01},

journal = {Journal of Food Processing and Preservation},

volume = {45},

issue = {9},

pages = {e15496},

publisher = {John Wiley & Sons, Ltd},

abstract = {Citrus canker is a quarentenary disease caused by Xanthomonas citri subsp. citri (X. citri). Thus, sanitization of fresh fruit is a necessary measure before any commercial activity. Therefore, we evaluated the clove essential oil (CEO), as an alternative sanitizer for the disinfection of citrus fruit in packinghouses. Tests in vitro and in vivo were carried out to determine the cell inhibitory concentration and to verify the efficacy of the oil for the disinfection of citrus fruits. In in vitro tests, CEO was able to inhibit X. citri when used at 0.75% (v/v). In experiments that simulate the sanitization process used in packinghouses, 5% CEO was as effective as the recommended sanitization product based on sodium hypochlorite. GC-MS results showed a high presence of eugenol derivatives as the major compounds of CEO. All results proved that CEO is a potential sanitizer that could be used as an alternative to sodium hypochlorite. Novelty impact statement: Experimental evidence shows that the clove essential oil (CEO) has the same sanitization efficacy as sodium hypochlorite, which makes of CEO an alternative sanitizer for the decontamination of citrus fruits to be exported to the European Union. CEO is a safer and more sustainable sanitizer for the postharvest disinfection of citrus fruit.},

keywords = {},

pubstate = {published},

tppubtype = {article}

}